Image Scanning Microscopy (ISM) can improve the effective spatial resolution of conventional confocal laser scanning microscopy to its theoretical limit, without compromising any of its functionalities.
Our team implemented a natural design of ISM based on a fast single-photon detector array which allows straightforward upgrade of confocal microscopy and provides access to the raw scanned images, also paving the way to adaptive reconstruction methods.
While standard all-optical implementations of ISM can reduce versatility, our solution enables for a direct combination with fluorescence spectroscopy techniques, such as fluorescence lifetime imaging and fluorescence correlation spectroscopy.
Push confocal microscopy resolution to its theoretical limit by achieving a 2-fold improvement.
Achieve the same resolution as ideal confocal at one-tenth the illumination intensity.
Collect photons in time-tag modality in order to perform super-resolution time-resolved imaging.