Image scanning microscopy (ISM) improves the spatial resolution
of conventional confocal laser-scanning microscopy, however, current implementations reduce versatility and restrict its
combination with fluorescence spectroscopy techniques, such as fluorescence lifetime.
Our team implemented a natural design of ISM based on a fast single-photon detector array, which allows straightforward upgrade of an existing confocal microscope without compromising any of its functionalities.
In contrast to all-optical implementations, our approach provides access to the raw scanned images, also opening the way to adaptive reconstruction methods, capable of considering different imaging conditios and distortions. Castello et al., Nat. Methods (2019).