About us

Genoa Instruments is a spin-off project of the Istituto Italiano di Tecnologia pioneering new technologies in the field of optical microscopy.
The company will develop and commercialize a new confocal microscope based on the Image Scanning Microscopy technique, which will allow to achieve both spatial and temporal super-resolution at the same time.
Our innovative optical module will constitute a new paradigm in the field of optical microscopy, potentially establishing as the next generation confocal microscope.
The microscope will be highly affordable and platform independent, allowing for tailored solutions as well as for the upgrade of existing widefield and confocal microscopes.

Background

Image
scanning
microscopy

Image scanning microscopy (ISM) improves the spatial resolution of conventional confocal laser-scanning microscopy, but current implementations reduce versatility and restrict its combination with fluorescence spectroscopy techniques, such as fluorescence lifetime.
Our team implemented a natural design of ISM based on a fast single-photon detector array, which allows straightforward upgrade of an existing confocal microscope, without compromising any of its functionalities.
In contrast to all-optical implementations, our approach provides access to the raw scanned images, also opening the way to adaptive reconstruction methods, capable of considering different imaging conditios and distortions. Castello et al., Nat. Methods (2019).

Selected publicationsmore_vert
Bibliographyclose

M. Castello et al., “A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM,” Nat. Methods, vol. 16, no. 2, pp. 175–178, 2019. Link.

J. Dreier et al., “Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo,” Nat. Commun., vol. 10, no. 1, p. 556, 2019. Link.

C. J. R. Sheppard, M. Castello, G. Tortarolo, G. Vicidomini, and A. Diaspro, “Image formation in image scanning microscopy, including the case of two-photon excitation,” J. Opt. Soc. Am. A, vol. 34, no. 8, pp. 1339–1350, Aug. 2017. Link.

M. Castello, C. J. R. Sheppard, A. Diaspro, and G. Vicidomini, “Image scanning microscopy with a quadrant detector,” Opt. Lett., vol. 40, no. 22, p. 5355, 2015. Link.

M. Castello, A. Diaspro, and G. Vicidomini, “Multi-images deconvolution improves signal-to-noise ratio on gated stimulated emission depletion microscopy,” Appl. Phys. Lett., vol. 105, no. 23, p. 234106, Dec. 2014. Link.

Background
Prism header
PRISM
Spatial resolution icon
Spatial resolution

A better look at your sample with improved spatial resolution while maintaining optical-sectioning capabilities.

SNR resolution icon
Signal to noise

Take advantage of the super-brightness effect in order to collect high-resolution images with unprecedented signal-to-noise ratio.

Temporal resolution icon
Temporal resolution

Explore temporal dimension by performing fluorescence lifetime imaging without compromise.

Features

  • Optical Module Icon

    A high-end tailored module
    for super resolution.

  • Compatibility Icon

    Maximum compatibility with
    existing scanning techniques.

  • Installation Icon

    Plug and Play installation
    and\or upgrade.

  • Autoalignment Icon

    Autocalibration and
    alignment diagnostic tools.

Configurations

Configurations

Our system can be provided as a STAND-ALONE module
or COUPLED to a regular microscope body.

Applications

Use your standard fluorescent dyes and image your sample with improved spatial resolution.

Take advantage of better SNR in order to image deeper in your sample.

Use 10 times lower illumination power (with respect to standard confocal) for less photodamage and photobleaching.

Explore temporal dimension and perform FLIM experiments.

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